P265 Fast-track biomarker “Anti-Exophiala IgG” in suspected Exophiala spp. infection

The melanized dimorphic fungus Exophiala dermatitidis is detected mainly in the respiratory tract of patients with CF. However, how to determine clinical manifestation and indication for treatment bronchopulmonary mycosis difficult have not been systematically established yet. To conduct a prospective cohort study lasting 12 months. 200 sputum-producing cystic fibrosis who were ≥6 years age attended CF center included. Serum was analysed tailored, indirect in-house ELISA detect anti-Exophiala dermatitidis-IgG reaction. Reactivity by measuring absorbance at 450 nm our laboratory Brandenburg an der Havel, which performed collaborative partner from Bilbao, Spain (PhD student Leire Martin-Souto). results sputum sample analysis divided into two groups. One group culturally positive (>1 sample) other negative. specific IgG response significantly higher (p < 0.001). When multiple serum samples same patient are available, chronological imaging all allows immune response, associated both development infection efficacy antifungal therapy, even if do produce often seen CFTRm treated patients.